Overview

  • Product name
    Anti-KLF17 antibody - C-terminal
    See all KLF17 primary antibodies
  • Description
    Rabbit polyclonal to KLF17 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KLF17 aa 250-279 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5JT82

  • Positive control
    • HeLa cells; HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ40160 antibody
    • KLF17 antibody
    • KLF17_HUMAN antibody
    • Krueppel like factor 17 antibody
    • Krueppel-like factor 17 antibody
    • Novel zinc finger protein antibody
    • RP4 675G8.1 antibody
    • RP4675G8.1 antibody
    • Zfp393 antibody
    • Zinc finger protein 393 antibody
    • ZNF393 antibody
    see all

Images

  • Flow cytometric analysis of Hela cells labeling KLF17 with ab174956 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

  • Anti-KLF17 antibody - C-terminal (ab174956) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size: 43 kDa

References

ab174956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (naive ESC)
Permeabilization
Yes - 0.1% Triton/PBS
Specification
naive ESC
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 13 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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