Overview

  • Product name

  • Description

    Rabbit polyclonal to KLF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human KLF2 aa 100-200 conjugated to keyhole limpet haemocyanin.
    Database link: Q9Y5W3

  • Positive control

    • This antibody gave a positive signal in Human Liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab139699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    Binds to the CACCC box in the beta-globin gene promoter and activates transcription.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • KLF 2 antibody
    • Klf2 antibody
    • KLF2_HUMAN antibody
    • Krueppel-like factor 2 antibody
    • Kruppel like factor 2 antibody
    • Kruppel like factor antibody
    • Kruppel like factor LKLF antibody
    • Kruppel-like factor 2 (lung) antibody
    • Lklf antibody
    • Lung krueppel like factor antibody
    • Lung krueppel-like factor antibody
    • Lung Kruppel like zinc finger transcription factor antibody
    see all

Images

  • Anti-KLF2 antibody (ab139699) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 8 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139699 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Xin Y  et al. Resveratrol improves uric acid-induced pancreatic ß-cells injury and dysfunction through regulation of miR-126. Biomed Pharmacother 102:1120-1126 (2018). WB . Read more (PubMed: 29710530) »
  • Rymut SM  et al. Improved Growth Patterns in Cystic Fibrosis Mice after Loss of Histone Deacetylase 6. Sci Rep 7:3676 (2017). WB . Read more (PubMed: 28623308) »
See all 3 Publications for this product

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