Product nameAnti-KLF2 antibody
See all KLF2 primary antibodies
DescriptionRabbit polyclonal to KLF2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Orangutan
Synthetic peptide corresponding to Human KLF2 aa 100-200 conjugated to keyhole limpet haemocyanin.
Database link: Q9Y5W3
- This antibody gave a positive signal in Human Liver tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
FunctionBinds to the CACCC box in the beta-globin gene promoter and activates transcription.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
modificationsUbiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
- Information by UniProt
- KLF 2 antibody
- Klf2 antibody
- KLF2_HUMAN antibody
Anti-KLF2 antibody (ab139699) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 8 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139699 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This product has been referenced in:
- Xin Y et al. Resveratrol improves uric acid-induced pancreatic ß-cells injury and dysfunction through regulation of miR-126. Biomed Pharmacother 102:1120-1126 (2018). WB . Read more (PubMed: 29710530) »
- Rymut SM et al. Improved Growth Patterns in Cystic Fibrosis Mice after Loss of Histone Deacetylase 6. Sci Rep 7:3676 (2017). WB . Read more (PubMed: 28623308) »