Overview

  • Product name
  • Description
    Goat polyclonal to KLF2
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide: ALSEPILPSFST, corresponding to N terminal amino acids 2-13 of Human KLF2.

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab17008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    ELISA: 1/32000.
    WB: Use at a concentration of 1.5 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa) from human heart lysates.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the CACCC box in the beta-globin gene promoter and activates transcription.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      modifications
      Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • KLF 2 antibody
      • Klf2 antibody
      • KLF2_HUMAN antibody
      • Krueppel-like factor 2 antibody
      • Kruppel like factor 2 antibody
      • Kruppel like factor antibody
      • Kruppel like factor LKLF antibody
      • Kruppel-like factor 2 (lung) antibody
      • Lklf antibody
      • Lung krueppel like factor antibody
      • Lung krueppel-like factor antibody
      • Lung Kruppel like zinc finger transcription factor antibody
      see all

    Images

    • ab17008 at 1.5µg/ml detecting KLF2 from human heart lysate by Western blot. 35µg of total protein was loaded per lane. Primary antibody was incubated for 1 hour. The blot was detected using chemiluminescence.

       

       

      ab17008 at 1.5µg/ml detecting KLF2 from human heart lysate by Western blot. 35µg of total protein was loaded per lane. Primary antibody was incubated for 1 hour. The blot was detected using chemiluminescence.

    References

    This product has been referenced in:
    • Zou K  et al. Krüppel-like factor 2 promotes cell proliferation in hepatocellular carcinoma through up-regulation of c-myc. Cancer Biol Ther 17:20-6 (2016). Read more (PubMed: 26853883) »
    • Tuomisto TT  et al. Simvastatin has an anti-inflammatory effect on macrophages via upregulation of an atheroprotective transcription factor, Kruppel-like factor 2. Cardiovasc Res 78:175-84 (2008). Read more (PubMed: 18192240) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E3.75 mouse blastocyst)
    Specification
    E3.75 mouse blastocyst

    Mr. Vidur Garg

    Verified customer

    Submitted May 30 2018

    Question
    Answer

    Thank you for your enquiry. Since this KLF2 is a transcription factor, it is expected to reside in the nucleus. Therefore, it will recognize the intracellular domain. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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    Question
    Answer

    Thank you for your enquiry. No, this has not been specifically tested. The sequence of the peptide used as the immunogen is listed on the online datasheet and you can BLAST it (the BLAST link is on the online datasheet too) to see if it is likely to cross-react with other KLFs. I tried it but no similarity came up. Please contact us again if you have any additional questions.

    Read More

    Answer

    Thank you for taking the time to send us your protocol details, this was very useful for me to understand your problem. We have not tested this antibody in the same samples as yours; in our hands, our positive control of human heart lysate gave a single band at the expect MW of 41kDa. Could you please clarify if you have tested in WB your samples for KLF2 before? I am wondering if the samples express a different form than the protein found in human heart lysate samples. Could you also tell me a little bit more about your blocking conditions: what you blocking with, for how long and if you added the blocking agent to the antibody dilution buffer. Sometimes blocking can prevent adequate binding of the antibody to the membrane and create non specific binding and/or no binding to the band of interest. We typically recommend a 1hr blocking step with 5%BSA or 5%milk in TBST and incubate the membrane with the primary and secondary antibodies diluted in TBST only. Please be assured that under the Abcam guarantee if you run our positive control in WB and find similar problems we will offer a replacement or refund if the antibody was purchased in the last 90days. I was unable to find an order with the reference C0144771, did you purchase the antibody through one of our distributors? This is not a problem if this is the case, we can liase with the distributor to arrange a refund or replacement if you find that the human heart lysate samples give problems too. I hope the above information will be useful, if you require further assistance please not hesitate to contact me,

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    Answer

    Thank you for your phone call and I'm sorry to hear that you are experiencing difficulty with ab17008. Would you be able to provide me with a detailed protocol? This will assist in helping to troubleshoot. It would be really helpful if you could click on the link below which will direct you to our WB questionnaire. Thanks again, and I look forward to hearing from you. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=17008&mode=questionaire

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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