Overview

  • Product name

  • Description

    Rabbit polyclonal to KLF4
  • Host species

    Rabbit
  • Specificity

    ab106629 will detect all three known isoforms. It does not cross-react with other KLF members.
  • Tested applications

    Suitable for: ChIP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human KLF4 (C terminal). 20 amino acid synthetic peptide located near the c-terminus.
    Database link: O43474

  • Positive control

    • WB: Human liver tissue lysate IHC-P: Mouse colon tissue, Rat liver tissue.
  • General notes

    For information on sample preparation for western blot, please refer here

Applications

Our Abpromise guarantee covers the use of ab106629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 25644173
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • Function

    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

Images

  • Lane 1 : Anti-KLF4 antibody (ab106629) at 1 µg/ml
    Lane 2 : Anti-KLF4 antibody (ab106629) at 2 µg/ml

    All lanes : Human liver tissue lysate (7.5 µL (2 mg/mL) per well)

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : anti-rabbit IgG-HRP conjugated secondary at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa


    Exposure time: 30 seconds


    Run in transfer buffer at 130V for 2 hours (or until the dye front is close to the bottom). 

    After transfer, wash membrane in 1xTBST. 

    Incubate the membrane with blocking buffer overnight at 4°C with gentle agitation. 

    Dilute ab106629 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab106629 overnight at 4oC. Wash 3 times x 10 minutes in washing buffer with gentle agitation.

    Dilute 1μL anti-rabbit IgG-HRP conjugated secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 hour at room temperature. Wash 3 times x 10 minutes in washing buffer with gentle agitation. 

    Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS.

    Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS.

    Blocking Buffer: 5% non-fat dry milk, TBS.

    Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20. 

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KLF4 with ab106629 at 5 µg/mL. Antigen Retrieval: Use Citrate buffer antigen retrieval using a microwave. Endogenous peroxide blocking with 3% H2O2. Blocking with 5% BSA at room temperature for 30 minutes. ab106629 incubation at 4°C overnight. Secondary antibody incubation at room temperature for 30 minutes. Washing 3 times for 5 minutes using phosphate buffered saline. Detection using DAB. 

  • ab106629 at 5 ug/ml, staining KLF4 in Rat liver by Immunohistochemistry, Paraffin-embedded tissue.

References

This product has been referenced in:

  • Li L  et al. Etv6 activates vegfa expression through positive and negative transcriptional regulatory networks in Xenopus embryos. Nat Commun 10:1083 (2019). WB, ChIP ; Xenopus laevis . Read more (PubMed: 30842454) »
  • Zhang N  et al. Downregulated Krüppel-like factor 4 expression is associated with the aggressiveness of prostate cancer. Oncol Rep 41:1789-1796 (2019). Read more (PubMed: 30747213) »
See all 10 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 15 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human iPS cells)
Permeabilization
Yes - 0.5% TritonX
Specification
human iPS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 15 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mESC CCE (feeder free))
Permeabilization
Yes - 0.5% Triton-X in PBS
Specification
mESC CCE (feeder free)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Paraformaldehyde

Ms. Joyce Noble

Verified customer

Submitted Feb 01 2019

Abreviews
Application
CyTOF®
Sample
Mouse Cell (Lung)
Specification
Lung

Irit Milman Krentsis

Verified customer

Submitted Nov 14 2017

Question

Catalog number(s): 72543

Product(s): Rabbit polyclonal to KLF4

Survey results
----------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. Yes

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I requested protocol advice
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

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Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. No

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Credit note / refund section
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Q. How satisfied are you with how the complaint was handled?
A.Very satisfied

Q. Could we have done anything to better resolve the problem?
A.Only perhaps recommended another product

Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.

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Answer

Thank you for your participation in Abcam's follow up survey.

I have checked for alternative antibodies that were tested in WB as well as with mouse:

ab106629:https://www.abcam.com/klf4-antibody-ab106629.html

We also have ab77090 which is predicted to react with mouse but not yet tested. In a case like this we offer our testing discount program to try the antibody. Please see below for more details.
https://www.abcam.com/klf4-antibody-ab77090.html

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

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