Product nameAnti-KLF4 antibody
See all KLF4 primary antibodies
DescriptionRabbit polyclonal to KLF4
Specificityab106629 will detect all three known isoforms. It does not cross-react with other KLF members.
Tested applicationsSuitable for: ChIP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human KLF4 (C terminal). 20 amino acid synthetic peptide located near the c-terminus.
Database link: O43474
- WB: Human liver tissue lysate IHC-P: Mouse colon tissue, Rat liver tissue.
For information on sample preparation for western blot, please refer here.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106629 is purified by affinity chromatography via a peptide column.
ChIP Related Products
Our Abpromise guarantee covers the use of ab106629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 25644173|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
- Information by UniProt
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
Lane 1 : Anti-KLF4 antibody (ab106629) at 1 µg/ml
Lane 2 : Anti-KLF4 antibody (ab106629) at 2 µg/ml
All lanes : Human liver tissue lysate (7.5 µL (2 mg/mL) per well)
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG-HRP conjugated secondary at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 30 seconds
Run in transfer buffer at 130V for 2 hours (or until the dye front is close to the bottom).
After transfer, wash membrane in 1xTBST.
Incubate the membrane with blocking buffer overnight at 4°C with gentle agitation.
Dilute ab106629 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab106629 overnight at 4oC. Wash 3 times x 10 minutes in washing buffer with gentle agitation.
Dilute 1μL anti-rabbit IgG-HRP conjugated secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 hour at room temperature. Wash 3 times x 10 minutes in washing buffer with gentle agitation.
Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS.
Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS.
Blocking Buffer: 5% non-fat dry milk, TBS.
Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KLF4 with ab106629 at 5 µg/mL. Antigen Retrieval: Use Citrate buffer antigen retrieval using a microwave. Endogenous peroxide blocking with 3% H2O2. Blocking with 5% BSA at room temperature for 30 minutes. ab106629 incubation at 4°C overnight. Secondary antibody incubation at room temperature for 30 minutes. Washing 3 times for 5 minutes using phosphate buffered saline. Detection using DAB.
ab106629 at 5 ug/ml, staining KLF4 in Rat liver by Immunohistochemistry, Paraffin-embedded tissue.
This product has been referenced in:
- Li L et al. Etv6 activates vegfa expression through positive and negative transcriptional regulatory networks in Xenopus embryos. Nat Commun 10:1083 (2019). WB, ChIP ; Xenopus laevis . Read more (PubMed: 30842454) »
- Zhang N et al. Downregulated Krüppel-like factor 4 expression is associated with the aggressiveness of prostate cancer. Oncol Rep 41:1789-1796 (2019). Read more (PubMed: 30747213) »