Key features and details
- Rabbit polyclonal to KLF4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KLF4 antibody
See all KLF4 primary antibodies
DescriptionRabbit polyclonal to KLF4
Specificityab106629 will detect all three known isoforms. It does not cross-react with other KLF members.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human KLF4 (C terminal). 20 amino acid synthetic peptide located near the c-terminus.
Database link: O43474
For information on sample preparation for western blot, please refer here.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106629 is purified by affinity chromatography via a peptide column.
ChIP Related Products
Our Abpromise guarantee covers the use of ab106629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
- Information by UniProt
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
Lane 1 : Anti-KLF4 antibody (ab106629) at 1 µg/ml
Lane 2 : Anti-KLF4 antibody (ab106629) at 2 µg/ml
All lanes : Human liver tissue lysate (7.5 µL (2 mg/mL) per well)
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG-HRP conjugated secondary at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 30 seconds
Run in transfer buffer at 130V for 2 hours (or until the dye front is close to the bottom).
After transfer, wash membrane in 1xTBST.
Incubate the membrane with blocking buffer overnight at 4°C with gentle agitation.
Dilute ab106629 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab106629 overnight at 4oC. Wash 3 times x 10 minutes in washing buffer with gentle agitation.
Dilute 1μL anti-rabbit IgG-HRP conjugated secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 hour at room temperature. Wash 3 times x 10 minutes in washing buffer with gentle agitation.
Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS.
Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS.
Blocking Buffer: 5% non-fat dry milk, TBS.
Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KLF4 with ab106629 at 5 µg/mL. Antigen Retrieval: Use Citrate buffer antigen retrieval using a microwave. Endogenous peroxide blocking with 3% H2O2. Blocking with 5% BSA at room temperature for 30 minutes. ab106629 incubation at 4°C overnight. Secondary antibody incubation at room temperature for 30 minutes. Washing 3 times for 5 minutes using phosphate buffered saline. Detection using DAB.
ab106629 has been referenced in 21 publications.
- Jin L et al. Kruppel-like factor 4 improves obesity-related nephropathy through increasing mitochondrial biogenesis and activities. J Cell Mol Med 24:1200-1207 (2020). PubMed: 31800161
- Jiang Z et al. Long non-coding RNA LINC00673 silencing inhibits proliferation and drug resistance of prostate cancer cells via decreasing KLF4 promoter methylation. J Cell Mol Med 24:1878-1892 (2020). PubMed: 31881124
- Sadhukhan R et al. Fractionated radiation suppresses Kruppel-like factor 2 pathway to a greater extent than by single exposure to the same total dose. Sci Rep 10:7734 (2020). PubMed: 32382091
- Karagonlar ZF et al. A Novel Function for KLF4 in Modulating the De-differentiation of EpCAM-/CD133- nonStem Cells into EpCAM+/CD133+ Liver Cancer Stem Cells in HCC Cell Line HuH7. Cells 9:N/A (2020). PubMed: 32408542
- Yang GS et al. Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. Theranostics 10:7787-7811 (2020). PubMed: 32685020