Overview

  • Product name

  • Description

    Rabbit polyclonal to KLF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: U-87, C2C12, THP-1, J774A.1, PC-3, HT-29, A549 and HeLa whole cell lysates. ICC/IF: PC-3 cells.

Applications

Our Abpromise guarantee covers the use of ab216875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

Images

  • Immunofluorescent analysis of 0.5% PFA-fixed (A and C) and 0.5% methanol-fixed (B and D) PC-3 (human prostate adenocarcinoma cell line) cells labeling KLF4 with ab216875 at 10 µg/ml (green).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: C and D.

  • All lanes : Anti-KLF4 antibody (ab216875) at 1/1000 dilution

    Lanes 1 & 13 : MW ladder (opal pre-stained)
    Lane 2 : U-87 whole cell lysate
    Lane 3 : C2C12 whole cell lysate
    Lane 4 : NIH/3T3 whole cell lysate
    Lane 5 : THP-1 whole cell lysate
    Lane 6 : J774A.1 whole cell lysate
    Lane 7 : PC-3 whole cell lysate
    Lane 8 : HT-29 whole cell lysate
    Lane 9 : Mouse colon lysate
    Lane 10 : A549 whole cell lysate
    Lane 11 : MOLT-4 whole cell lysate
    Lane 12 : HeLa whole cell lysate

    Secondary
    All lanes : Rabbit secondary HRP antibody at 1/70000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking buffer: Blotto 5%.

    Dilution buffer: 1X PBS, 0.01% azide.

References

ab216875 has not yet been referenced specifically in any publications.

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