Product nameAnti-KLF4 antibody
See all KLF4 primary antibodies
DescriptionRabbit polyclonal to KLF4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: U-87, C2C12, THP-1, J774A.1, PC-3, HT-29, A549 and HeLa whole cell lysates. ICC/IF: PC-3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab216875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
- Information by UniProt
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
Immunofluorescent analysis of 0.5% PFA-fixed (A and C) and 0.5% methanol-fixed (B and D) PC-3 (human prostate adenocarcinoma cell line) cells labeling KLF4 with ab216875 at 10 µg/ml (green).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: C and D.
All lanes : Anti-KLF4 antibody (ab216875) at 1/1000 dilution
Lanes 1 & 13 : MW ladder (opal pre-stained)
Lane 2 : U-87 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lane 6 : J774A.1 whole cell lysate
Lane 7 : PC-3 whole cell lysate
Lane 8 : HT-29 whole cell lysate
Lane 9 : Mouse colon lysate
Lane 10 : A549 whole cell lysate
Lane 11 : MOLT-4 whole cell lysate
Lane 12 : HeLa whole cell lysate
All lanes : Rabbit secondary HRP antibody at 1/70000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking buffer: Blotto 5%.
Dilution buffer: 1X PBS, 0.01% azide.
ab216875 has not yet been referenced specifically in any publications.