Product nameAnti-KLF4 antibody
See all KLF4 primary antibodies
DescriptionRabbit polyclonal to KLF4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human colon carcinoma lysate and tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 55 kDa).|
FunctionTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
- Information by UniProt
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue, labeling KLF4 using ab216968 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-KLF4 antibody (ab216968) at 1/200 dilution
Lane 1 : rat kidney lysate
Lane 2 : Human colon carcinoma lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
ab216968 has not yet been referenced specifically in any publications.