Anti-KLF4 antibody (ab272860)
Overview
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Product name
Anti-KLF4 antibody
See all KLF4 primary antibodies -
Description
Rabbit polyclonal to KLF4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human KLF4 (C terminal). The exact sequence is proprietary.
Database link: O43474 -
Positive control
- Flow Cyt: Human embryonic stem cells (ESC). WB: HEK-293T, A431, HeLa, HepG2, HCT 116, Rat2, NIH/3T3 whole cell lysate and mouse and human ESC. IP: HeLa whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab272860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50 - 1/200. | |
| WB | 1/500 - 1/3000. | |
| IP | 1/100 - 1/500. |
Target
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Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9314 Human
- Entrez Gene: 16600 Mouse
- Entrez Gene: 114505 Rat
- Omim: 602253 Human
- SwissProt: O43474 Human
- SwissProt: Q60793 Mouse
- Unigene: 376206 Human
- Unigene: 4325 Mouse
see all -
Alternative names
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
see all
Images
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Western blot - Anti-KLF4 antibody (ab272860)All lanes : Anti-KLF4 antibody (ab272860) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Flow Cytometry - Anti-KLF4 antibody (ab272860)Flow cytometry analysis of human embryonic stem cells labeling KLF4 using ab272860 at 1:100 dilution (purple) or rabbit IgG (black).
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Western blot - Anti-KLF4 antibody (ab272860)All lanes : Anti-KLF4 antibody (ab272860) at 1/2000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 nuclear extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-Synaptojanin antibody (ab272560) at 1/5000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-KLF4 antibody (ab272860) at 1/5000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-KLF4 antibody (ab272860) at 1/3000 dilution + Mouse embryonic stem cells at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-KLF4 antibody (ab272860) at 1/3000 dilution + Human embryonic stem cells at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)All lanes : Anti-KLF4 antibody (ab272860) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Transfected HEK-293 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Immunoprecipitation - Anti-KLF4 antibody (ab272860)10% SDS-PAGE
All lanes : Anti-KLF4 antibody (ab272860) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Control IgG
Lane 3 : Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 µg of ab272860
Secondary
All lanes : anti Rabbit IgG (HRP)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab272860 has not yet been referenced specifically in any publications.
