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  1. Link

    klf4-antibody-ab272860.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-KLF4 antibody (ab272860)

  • Datasheet
  • SDS
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Western blot - Anti-KLF4 antibody (ab272860)
  • Flow Cytometry - Anti-KLF4 antibody (ab272860)
  • Western blot - Anti-KLF4 antibody (ab272860)
  • Western blot - Anti-KLF4 antibody (ab272860)
  • Western blot - Anti-KLF4 antibody (ab272860)
  • Western blot - Anti-KLF4 antibody (ab272860)
  • Western blot - Anti-KLF4 antibody (ab272860)
  • Western blot - Anti-KLF4 antibody (ab272860)
  • Immunoprecipitation - Anti-KLF4 antibody (ab272860)

Key features and details

  • Rabbit polyclonal to KLF4
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KLF4 antibody
    See all KLF4 primary antibodies
  • Description

    Rabbit polyclonal to KLF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KLF4 (C terminal). The exact sequence is proprietary.
    Database link: O43474

  • Positive control

    • Flow Cyt: Human embryonic stem cells (ESC). WB: HEK-293T, A431, HeLa, HepG2, HCT 116, Rat2, NIH/3T3 whole cell lysate and mouse and human ESC. IP: HeLa whole cell extracts.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50 - 1/200.
WB
1/500 - 1/3000.
IP
1/100 - 1/500.
Notes
Flow Cyt
1/50 - 1/200.
WB
1/500 - 1/3000.
IP
1/100 - 1/500.

Target

  • Function

    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O43474 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9314 Human
    • Entrez Gene: 16600 Mouse
    • Entrez Gene: 114505 Rat
    • Omim: 602253 Human
    • SwissProt: O43474 Human
    • SwissProt: Q60793 Mouse
    • Unigene: 376206 Human
    • Unigene: 4325 Mouse
    • Alternative names

      • Endothelial Kruppel like zinc finger protein antibody
      • Epithelial zinc finger protein EZF antibody
      • EZF antibody
      • GKLF antibody
      • gut Kruppel-like factor antibody
      • Gut-enriched krueppel-like factor antibody
      • KLF antibody
      • KLF4 antibody
      • KLF4_HUMAN antibody
      • Krueppel-like factor 4 antibody
      • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
      • Kruppel like factor 4 (gut) antibody
      see all

    Images

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      All lanes : Anti-KLF4 antibody (ab272860) at 1/5000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody


      10% SDS-PAGE

    • Flow Cytometry - Anti-KLF4 antibody (ab272860)
      Flow Cytometry - Anti-KLF4 antibody (ab272860)

      Flow cytometry analysis of human embryonic stem cells labeling KLF4 using ab272860 at 1:100 dilution (purple) or rabbit IgG (black).

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      All lanes : Anti-KLF4 antibody (ab272860) at 1/2000 dilution

      Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
      Lane 2 : HCT 116 nuclear extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody


      10% SDS-PAGE

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      Anti-Synaptojanin antibody (ab272560) at 1/5000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG antibody


      10% SDS-PAGE

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      Anti-KLF4 antibody (ab272860) at 1/5000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

      Secondary
      HRP-conjugated anti-rabbit IgG antibody


      10% SDS-PAGE

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      Anti-KLF4 antibody (ab272860) at 1/3000 dilution + Mouse embryonic stem cells at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG antibody


      12% SDS-PAGE

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      Anti-KLF4 antibody (ab272860) at 1/3000 dilution + Human embryonic stem cells at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG antibody


      10% SDS-PAGE

    • Western blot - Anti-KLF4 antibody (ab272860)
      Western blot - Anti-KLF4 antibody (ab272860)
      All lanes : Anti-KLF4 antibody (ab272860) at 1/1000 dilution

      Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : Transfected HEK-293 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody


      10% SDS-PAGE

    • Immunoprecipitation - Anti-KLF4 antibody (ab272860)
      Immunoprecipitation - Anti-KLF4 antibody (ab272860)

      10% SDS-PAGE.

      Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of ab272860.



      All lanes : Anti-KLF4 antibody (ab272860) at 1/500 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : Control IgG
      Lane 3 : Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 µg of ab272860

      Secondary
      All lanes : anti Rabbit IgG (HRP)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab272860? Please let us know so that we can cite the reference in this datasheet.

    ab272860 has not yet been referenced specifically in any publications.

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