Key features and details
- Rabbit polyclonal to KLF5
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLF5 antibody
See all KLF5 primary antibodies
DescriptionRabbit polyclonal to KLF5
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human KLF5 aa 341-370 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q13887
- IHC-P: Human kidney tissue. ICC/IF: U-251 MG cells. WB: CCRF-CEM cell lysate. Flow Cyt: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab230861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.|
|IHC-P||1/10 - 1/100.|
|WB||1/1000. Predicted molecular weight: 51 kDa.|
FunctionTranscription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Tissue specificityExpressed only in testis and placenta.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
modificationsUbiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
- Information by UniProt
FormKLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
- Basic transcription element binding protein 2 antibody
- Basic transcription element-binding protein 2 antibody
- BTE binding protein 2 antibody
Anti-KLF5 antibody (ab230861) at 1/1000 dilution + CCRF-CEM (Human T cell lymphoblast cell line) cell lysate at 35 µg
Predicted band size: 51 kDa
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KLF5 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling KLF5 (green) using ab230861 at 1/10 dilution in ICC/IF.
For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
Formalin-fixed, paraffin-embedded human kidney tissue stained for KLF5 using ab230861 at 1/10 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230861 has not yet been referenced specifically in any publications.