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Synthetic peptide within Human KLF5 aa 341-370 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q13887
Our Abpromise guarantee covers the use of ab230861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.|
|IHC-P||1/10 - 1/100.|
|WB||1/1000. Predicted molecular weight: 51 kDa.|
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KLF5 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling KLF5 (green) using ab230861 at 1/10 dilution in ICC/IF.
For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
Formalin-fixed, paraffin-embedded human kidney tissue stained for KLF5 using ab230861 at 1/10 dilution in immunohistochemical analysis.
ab230861 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"