Overview

  • Product name
  • Description
    Rabbit polyclonal to KLF5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human KLF5 aa 341-370 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q13887

  • Positive control
    • IHC-P: Human kidney tissue. ICC/IF: U-251 MG cells. WB: CCRF-CEM cell lysate. Flow Cyt: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.
IHC-P 1/10 - 1/100.
WB 1/1000. Predicted molecular weight: 51 kDa.

Target

  • Function
    Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
  • Tissue specificity
    Expressed only in testis and placenta.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
  • Alternative names
    • Basic transcription element binding protein 2 antibody
    • Basic transcription element-binding protein 2 antibody
    • BTE binding protein 2 antibody
    • BTE-binding protein 2 antibody
    • BTEB 2 antibody
    • BTEB2 antibody
    • CKLF antibody
    • Colon krueppel like factor antibody
    • Colon krueppel-like factor antibody
    • GC box binding protein 2 antibody
    • GC-box-binding protein 2 antibody
    • IKLF antibody
    • Intestinal enriched krueppel like factor antibody
    • Intestinal-enriched krueppel-like factor antibody
    • KLF 5 antibody
    • Klf5 antibody
    • KLF5_HUMAN antibody
    • Krueppel like factor 5 antibody
    • Krueppel-like factor 5 antibody
    • Kruppel like factor 5 intestinal antibody
    • Transcription factor BTEB 2 antibody
    • Transcription factor BTEB2 antibody
    see all

Images

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KLF5 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-KLF5 antibody (ab230861) at 1/1000 dilution + CCRF-CEM (Human T cell lymphoblast cell line) cell lysate at 35 µg

    Predicted band size: 51 kDa

  • 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling KLF5 (green) using ab230861 at 1/10 dilution in ICC/IF.

    For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for KLF5 using ab230861 at 1/10 dilution in immunohistochemical analysis.

References

ab230861 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Mouse Embryo (E3.75 mouse blastocyst)
Specification
E3.75 mouse blastocyst

Mr. Vidur Garg

Verified customer

Submitted May 30 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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