Anti-KLF5 antibody (ab24331)

Reviews (6)Q&A (9)References (23)

Overview

  • Product name
    Anti-KLF5 antibody
    See all KLF5 primary antibodies
  • Description
    Rabbit polyclonal to KLF5
  • Host species
    Rabbit
  • Specificity
    Some of our customers have successfully used ab24331 on mouse, please see PubMed ID: 19720868 and 21600198. However, we have been unable to show cross-reactivity with this species and therefore we cannot guarantee this product will consistently work on mouse. Please contact our Scientific Support team for further information.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF5.

    Read Abcam's proprietary immunogen policy (Peptide available as ab30882.)

  • Positive control
    • This antibody gave a positive signal in Human small intestine tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab24331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.6 - 3 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21600198
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
  • Tissue specificity
    Expressed only in testis and placenta.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Form
      KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
    • Alternative names
      • Basic transcription element binding protein 2 antibody
      • Basic transcription element-binding protein 2 antibody
      • BTE binding protein 2 antibody
      • BTE-binding protein 2 antibody
      • BTEB 2 antibody
      • BTEB2 antibody
      • CKLF antibody
      • Colon krueppel like factor antibody
      • Colon krueppel-like factor antibody
      • GC box binding protein 2 antibody
      • GC-box-binding protein 2 antibody
      • IKLF antibody
      • Intestinal enriched krueppel like factor antibody
      • Intestinal-enriched krueppel-like factor antibody
      • KLF 5 antibody
      • Klf5 antibody
      • KLF5_HUMAN antibody
      • Krueppel like factor 5 antibody
      • Krueppel-like factor 5 antibody
      • Kruppel like factor 5 intestinal antibody
      • Transcription factor BTEB 2 antibody
      • Transcription factor BTEB2 antibody
      see all

    Images

    • Western blot - Anti-KLF5 antibody (ab24331)
      Western blot - Anti-KLF5 antibody (ab24331)
      Anti-KLF5 antibody (ab24331) at 2 µg/ml + Small Intestine (Human) Tissue Lysate at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 50 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab24331)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab24331)
      The image shows nuclear staining of the epithelial cells of the intestinal gland. Tris EDTA pH9 and Citrate pH6 are both suitable antigen retrieval techniques.
    • Western blot - Anti-KLF5 antibody (ab24331)
      Western blot - Anti-KLF5 antibody (ab24331)This image is courtesy of an anonymous Abreview
      All lanes : Anti-KLF5 antibody (ab24331) at 1/500 dilution

      Lane 1 : Marker ladder
      Lane 2 : Cell lysate prepared from human Ls174T cells at 40 µg
      Lane 3 : Cell lysate prepared from IEC-6 rat intestinal cells at 40 µg

      Secondary
      Lanes 2-3 : HRP conjugated goat polyclonal to rabbit IgG at 1/1500 dilution

      Predicted band size: 50 kDa


      Exposure time: 2 minutes

      See Abreview

    References

    This product has been referenced in:
    See all 23 Publications for this product

    Publishing research using ab24331? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 15 Abreviews or Q&A

    Question

    Hello, The confirmation # is and the purchase order # is Thank you,

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1040220.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    IHC - Wholemount abreview for Anti-KLF5 antibody

     Poor
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E3.75 mouse blastocyst)
    Specification
    E3.75 mouse blastocyst
    Read More

    Mr. Vidur Garg

    Verified customer

    Submitted May 30 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KLF5 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human ESC)
    Permeabilization
    Yes - 0.5% Triton X in PBS
    Specification
    human ESC
    Blocking step
    10% Donkey serum in PBS + 0.1% Tween 20 for 30 minutes at room temperature as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 14 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-KLF5 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (adult testes)
    Specification
    adult testes
    Permeabilization
    No
    Fixative
    4%PFA
    Read More

    Dr. Qing Wen

    Verified customer

    Submitted May 01 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KLF5 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
    Sample
    Mouse Tissue sections (adult testes)
    Specification
    adult testes
    Permeabilization
    No
    Fixative
    4%PFA
    Read More

    Dr. Qing Wen

    Verified customer

    Submitted May 01 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KLF5 antibody

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Airway epithelial cells)
    Specification
    Airway epithelial cells
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Read More

    Prof. Marc Chanson

    Verified customer

    Submitted Sep 07 2012

    Question

    Thank you for contacting me. We had tried the Klf5 Ab on mouse samples
    and it did not work.
    If you could replace this with a different Ab that would be great.
    We would like Anti-LBX1 antibody (ab90839)
    Let me know if this is feasible.
    This was the order info:
    Order details for xxx

    Read More
    Answer

    Thank you for your email.

    I am sorry this product did not perform in mouseand for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for ab90839. The item is currently backordered, and the estimated delivery date is xxx. I hope this won't be a problem.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    I recently purchased the above mentioned antibody for use with mouse, is it possible to get a replacement antibody or credit note.

    Read More
    Answer

    Thank you for contacting us.

    Yes, I would be happy to send you either a free of charge replacement or a credit. Please let me know what you prefer. Which antibody I should send orwhether a credit note would be best.

    I look forward to hear back from you.

    Read More

    Question

    We did indeed purchase this antibody for use in mouse samples and after extensive testing and use, were unable to detect any reactivity in lung issue. We now know why this occurred. You might also want to reevaluate the RFP antibody for use in mice, ab62341. We had invested considerable time and resources investigating these antibodies and wish to receive compensation.

    We currently use several antibodies from Abcam.
    We would like ab51317 and ab13970 to be sent to us.

    Read More
    Answer

    Thank you for your reply.

    I'd be happy to replace ab24331. Which of the 2 antibodies (ab51317 and ab13970) would you like me to send?

    As for ab62341 (which is not affected by the product information sent out for ab24331), we would require additionalinformation about the experiment, such as:

    1) What application was used?
    2) What was the sample type? How was it prepared?
    3) What was the exact problem?
    4) Whichantibody dilutions/incubation times were used?
    5) What was used as detection system and as blocking reagent?
    6) What is your orderor PO number for ab62341?
    This antibody is covered under our Abpromise guarantee for 6 months after purchase if used in the tested applications as listed on the datasheet.

    I look forward to hear back from you and assist you further.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Thank you so much for your email. We ordered this product on xxx. The order ID is xxx. I am not quite sure for the order number now. If possible, we would like to get a refund from abcam. Thank you so much for your great help and support. We love abcam's antibody which help our researches so much!!

    Read More
    Answer

    Thank you for contacting us.
    Your credit note ID for the refund is xxx.
    I am sorry that this antibody does not work in mouse as it had been stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
    The credit note ID is for your reference only and does not automatically guarantee the credit.
    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    1-10 of 15 Abreviews or Q&A

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