- Datasheet
- Specific References (22)
- Protocols
Overview
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Product nameAnti-KLF5 antibody
See all KLF5 primary antibodies -
DescriptionRabbit polyclonal to KLF5
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Host speciesRabbit
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SpecificitySome of our customers have successfully used ab24331 on mouse, please see PubMed ID: 19720868 and 21600198. However, we have been unable to show cross-reactivity with this species and therefore we cannot guarantee this product will consistently work on mouse. Please contact our Scientific Support team for further information.
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Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF5.
(Peptide available as ab30882.)
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Positive control
- This antibody gave a positive signal in Human small intestine tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab24331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.6 - 3 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21600198 | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
Target
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FunctionTranscription factor that binds to GC box promoter elements. Activates the transcription of these genes.
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Tissue specificityExpressed only in testis and placenta.
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Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsUbiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 688 Human
- Entrez Gene: 84410 Rat
- Omim: 602903 Human
- SwissProt: Q13887 Human
- Unigene: 508234 Human
- Unigene: 8954 Rat
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FormKLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation.
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Alternative names
- Basic transcription element binding protein 2 antibody
- Basic transcription element-binding protein 2 antibody
- BTE binding protein 2 antibody
see all
Images
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Western blot - Anti-KLF5 antibody (ab24331)Anti-KLF5 antibody (ab24331) at 2 µg/ml + Small Intestine (Human) Tissue Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab24331)The image shows nuclear staining of the epithelial cells of the intestinal gland. Tris EDTA pH9 and Citrate pH6 are both suitable antigen retrieval techniques. -
Western blot - Anti-KLF5 antibody (ab24331)This image is courtesy of an anonymous AbreviewAll lanes : Anti-KLF5 antibody (ab24331) at 1/500 dilution
Lane 1 : Marker ladder
Lane 2 : Cell lysate prepared from human Ls174T cells at 40 µg
Lane 3 : Cell lysate prepared from IEC-6 rat intestinal cells at 40 µg
Secondary
Lanes 2-3 : HRP conjugated goat polyclonal to rabbit IgG at 1/1500 dilution
Predicted band size: 50 kDa
Exposure time: 2 minutes
References
This product has been referenced in:
- Maroni G et al. Prep1 prevents premature adipogenesis of mesenchymal progenitors. Sci Rep 7:15573 (2017). Read more (PubMed: 29138456) »
- Liu JY et al. MicroRNA-153 inhibits the proliferation and invasion of human laryngeal squamous cell carcinoma by targeting KLF5. Exp Ther Med 11:2503-2508 (2016). WB ; Human . Read more (PubMed: 27284339) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"