Overview

  • Product name
  • Description
    Goat polyclonal to KLF8
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide:

    VDMDKLINNLEVQ

    , corresponding to N terminal amino acids 2-14 of Human KLF8.

  • Positive control
    • Human Kidney lysate.
  • General notes
    Principal Names – KLF8; Kruppel-like factor 8; BKLF3; DXS741; ZNF741; zinc finger protein 741. Official Gene Symbol - KLF8. GenBank Accession Number – NP_009181. LocusLink ID - 11279 (human)

Properties

Applications

Our Abpromise guarantee covers the use of ab2747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic krueppel-like factor 3 antibody
    • BKLF3 antibody
    • DXS741 antibody
    • KLF8 antibody
    • KLF8_HUMAN antibody
    • Krueppel-like factor 8 antibody
    • Kruppel like factor 8 antibody
    • MGC138314 antibody
    • Zinc finger protein 741 antibody
    • ZNF741 antibody
    see all

Images

  • Anti-KLF8 antibody (ab2747) at 2 µg/ml + 30ug human kidney lysate

    Predicted band size: 40 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    ab2747 staining (2µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

References

ab2747 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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