Anti-KLF8 antibody - N-terminal (ab229674)
Key features and details
- Rabbit polyclonal to KLF8 - N-terminal
- Suitable for: IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-KLF8 antibody - N-terminal
See all KLF8 primary antibodies -
Description
Rabbit polyclonal to KLF8 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC - Wholemountmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish KLF8 (N terminal). The exact sequence is proprietary.
Database link: A0S0R1 -
Positive control
- IHC - Wholemount: 1 day-post-fertilization zebrafish embryo.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab229674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC - Wholemount | 1/50 - 1/500. |
Target
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Function
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsSumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Basic krueppel-like factor 3 antibody
- BKLF3 antibody
- DXS741 antibody
see all
Images
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IHC - Wholemount - Anti-KLF8 antibody - N-terminal (ab229674)Whole mount 1 day-post-fertilization zebrafish embryo tissue stained for KLF8 using ab229674 at 1/50 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229674 has not yet been referenced specifically in any publications.
