Key features and details
- Rabbit polyclonal to KLF9
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLF9 antibody
See all KLF9 primary antibodies
DescriptionRabbit polyclonal to KLF9
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
- Jurkat cell lysate and Human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPeptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab26074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionTranscription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box.
Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- Basic transcription element binding protein 1 antibody
- Basic transcription element-binding protein 1 antibody
- BTE binding protein 1 antibody
Anti-KLF9 antibody (ab26074) at 0.5 µg/ml (in 5% skim milk / PBS buffer) +
Jurkat whole cell lysate (ab7899)
HRP conjugated anti-Rabbit IgG
Predicted band size: 27 kDa
Observed band size: 27,42 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemical detection of KLF9 using 4 ug/ml ab26074 in paraffin embedded human kidney tissue section. Arrows indicate positively labelled epithelial cells of the renal tubule.
ICC/IF image of ab26074 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26074, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab26074 has not yet been referenced specifically in any publications.