Overview

  • Product name

    Anti-KLH antibody (FITC)
    See all KLH primary antibodies
  • Description

    Rabbit polyclonal to KLH (FITC)
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Full length protein KLH (Keyhole Limpet Hemocyanin)

  • General notes

    3.4 moles FITC per mole of rabbit IgG.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.15% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab34766 was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab34766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
  • Cellular localization

    Secreted protein; extracellular space
  • Alternative names

    • Hemocyanin 2 c chain antibody
    • Hemocyanin 2c chain antibody
    • Hemocyanin A chain antibody
    • Hemocyanin B chain antibody
    • Keyhole limpet hemocyanin antibody
    • KLH A antibody
    • KLH B antibody
    • KLH2 c antibody
    • KLH2c antibody
    • KLHA antibody
    • KLHB antibody
    see all

References

This product has been referenced in:

  • Huang EY  et al. Amifostine alleviates radiation-induced lethal small bowel damage via promotion of 14-3-3s-mediated nuclear p53 accumulation. Oncotarget 5:9756-69 (2014). Flow Cyt . Read more (PubMed: 25230151) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your email.

Unfortunately, we are not able to offer small sample sizes of our product due to operational reasons. However, we guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

And for untested species and applications, we offer the testing discount program, I had emailed you about recently.

For bulk orders of different products, please contact our customer service team atmailto:us.orders@abcam.comorby phone at 1-888-772-2226. For bulk orders of the same item (large quantities), please contact sales@abcam.com and my colleague will be able to assist you further.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab34766 in ELISA. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ELISAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

Yes, ab47052 would bind to F(ab')2 since this antibody reacts with the heavy chains on Mouse IgG and with light chains common to most mouse immunoglobulins. The F(ab')2 fragment contains the light chains and part of the heavy chains.

The secondary antibody would be fine to use in flow cytometry and ELISA and we would guarantee it for these applications. I assume you have a plate reader for ELISA that covers the wavelength range?

The primary antibody has only been tested in flow cytometry, but not yet ELISA.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

Please let me know if I shall send you a testing discount code for trying ab34766 in ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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