Overview

  • Product name

    Anti-KLHDC8B antibody
  • Description

    Goat polyclonal to KLHDC8B
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Chinese hamster
  • Immunogen

    Synthetic peptide:

    C-TKLPRSLRMRDK

    , corresponding to internal sequence amino acids 259-270 of Human KLHDC8B according to NP_775817.1.

  • Positive control

    • Daudi and Jurkat cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Involvement in disease

    Defects in KLHDC8B are a cause of Hodgkin lymphoma (HL) [MIM:236000]. A malignant disease characterized by progressive enlargement of the lymph nodes, spleen and general lymphoid tissue, and the presence of large, usually multinucleate, cells (Reed-Sternberg cells). Reed-Sternberg cells compose only 1-2% of the total tumor cell mass. The remainder is composed of a variety of reactive, mixed inflammatory cells consisting of lymphocytes, plasma cells, neutrophils, eosinophils and histiocytes. Note=A chromosomal aberration disrupting KLHDC8B has been found in a family with the nodular sclerosis type of classic Hodgkin lymphoma. Translocation t(2,3)(q11.2;p21.31).
  • Sequence similarities

    Contains 8 Kelch repeats.
  • Developmental stage

    Transcribed predominantly during S phase, translated exclusively during mitosis and cytokinesis and rapidly degraded after cell division.
  • Cellular localization

    Cytoplasm. In mitotic cells, concentrates in the midbody of the cytoplasmic bridge linking daughter cells as they are about to separate during cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ11302 antibody
    • FP17659 antibody
    • Kelch domain containing 8B antibody
    • Kelch domain containing protein 8B antibody
    • Kelch domain-containing protein 8B antibody
    • KLD8B_HUMAN antibody
    • Klhdc8b antibody
    • MGC35097 antibody
    • MGC94736 antibody
    see all

Images

  • Anti-KLHDC8B antibody (ab128812) at 0.5 µg/ml + Daudi cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide

References

ab128812 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab128812.
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