Anti-KLHDC9 antibody (ab243586)
Key features and details
- Rabbit polyclonal to KLHDC9
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLHDC9 antibody -
Description
Rabbit polyclonal to KLHDC9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human KLHDC9 aa 179-250.
Sequence:QEGCHTASRSGHCAALLQTPGPHPGHQLLLFGGCNLAEPEVAGHWSHGKI KEEPPVAPHLMEQLARLVSSGQ
Database link: Q8NEP7 -
Positive control
- WB: HEK-293T overexpressing KLHDC9 cell lysate. IHC-P: Human esophagus tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab243586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Contains 3 Kelch repeats. - Information by UniProt
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Database links
- Entrez Gene: 126823 Human
- SwissProt: Q8NEP7 Human
- Unigene: 507290 Human
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Alternative names
- KARCA1 antibody
- Kelch domain containing 9 antibody
- Kelch domain containing protein 9 antibody
see all
Images
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Western blot - Anti-KLHDC9 antibody (ab243586)All lanes : Anti-KLHDC9 antibody (ab243586) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T overexpressing KLHDC9, cell lysate. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHDC9 antibody (ab243586)Formalin-fixed, paraffin-embedded human esophagus tissue stained for KLHDC9 using ab243586 at 1/200 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab243586 has not yet been referenced specifically in any publications.
