Anti-KLHDC9 antibody (ab243586)

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Overview

  • Product name

    Anti-KLHDC9 antibody

  • Description

    Rabbit polyclonal to KLHDC9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human KLHDC9 aa 179-250.
    Sequence:

    QEGCHTASRSGHCAALLQTPGPHPGHQLLLFGGCNLAEPEVAGHWSHGKI KEEPPVAPHLMEQLARLVSSGQ


    Database link: Q8NEP7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T overexpressing KLHDC9 cell lysate. IHC-P: Human esophagus tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab243586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml.

Target

  • Sequence similarities

    Contains 3 Kelch repeats.
  • Information by UniProt

  • Database links

    • Alternative names

      • KARCA1 antibody
      • Kelch domain containing 9 antibody
      • Kelch domain containing protein 9 antibody
      • Kelch domain-containing protein 9 antibody
      • Kelch/ankyrin repeat containing cyclin A1 interacting protein antibody
      • Kelch/ankyrin repeat-containing cyclin A1-interacting protein antibody
      • KLDC9_HUMAN antibody
      • Klhdc9 antibody
      • MGC33338 antibody
      • RP11-544M22.9 antibody
      see all

    Images

    • Western blot - Anti-KLHDC9 antibody (ab243586)
      Western blot - Anti-KLHDC9 antibody (ab243586)
      All lanes : Anti-KLHDC9 antibody (ab243586) at 0.4 µg/ml

      Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : HEK-293T overexpressing KLHDC9, cell lysate.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHDC9 antibody (ab243586)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHDC9 antibody (ab243586)

      Formalin-fixed, paraffin-embedded human esophagus tissue stained for KLHDC9 using ab243586 at 1/200 dilution in immunohistochemical analysis.

    References

    ab243586 has not yet been referenced specifically in any publications.

    Publishing research using ab243586? Please let us know so that we can cite the reference in this datasheet.

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