Overview

  • Product name
    Anti-KLHL1 antibody [EPR11506]
    See all KLHL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11506] to KLHL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KLHL1 aa 100-200. The exact sequence is proprietary.
    Database link: Q9NR64

  • Positive control
    • Human fetal brain, Human cerebellum, SH-SY5Y and HeLa lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11506
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Predicted molecular weight: 82 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • Flow cytometry analysis of permeabilized HeLa cells labeling KLHL1 (red), using ab170961 at a 1/10 dilution and negative control cells probed with a rabbit IgG (green)

    • All lanes : Anti-KLHL1 antibody [EPR11506] (ab170961) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human cerebellum lysate
      Lane 3 : SH-SY5Y lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 82 kDa

    References

    ab170961 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170961.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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