Overview

  • Product name

  • Description

    Rabbit polyclonal to KLHL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Recombinant fragment within Human KLHL2 (internal sequence). The exact sequence is proprietary.
    Database link: O95198

  • Positive control

    • WB: HEK-293T and A431 whole cell lysates. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play a role in organizing the actin cytoskeleton of the brain cells.
  • Tissue specificity

    Predominantly expressed in brain.
  • Sequence similarities

    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABP KELCH antibody
    • Actin binding protein Mayven antibody
    • Actin-binding protein Mayven antibody
    • kelch antibody
    • kelch like 2 antibody
    • Kelch like 2, Mayven antibody
    • Kelch like family member 2 antibody
    • Kelch like protein 2 antibody
    • Kelch-like protein 2 antibody
    • KLHL2 antibody
    • KLHL2_HUMAN antibody
    • MAV antibody
    • MAYVEN antibody
    • OTTHUMP00000219997 antibody
    • OTTHUMP00000219998 antibody
    see all

Images

  • All lanes : Anti-KLHL2 antibody (ab227539) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for KLHL2 with ab227539 at 1/500 dilution in immunohistochemical analysis.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KLHL2 (green) using ab227539 at 1/500 dilution in ICC/IF.

    Alpha-tubulin filaments were labeled with an anti-alpha-Tubulin antibody at 1/2000 dilution (red).

References

ab227539 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227539.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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