Key features and details
- Rabbit polyclonal to KLHL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLHL2 antibody
See all KLHL2 primary antibodies
DescriptionRabbit polyclonal to KLHL2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken
Recombinant fragment within Human KLHL2 (internal sequence). The exact sequence is proprietary.
Database link: O95198
- WB: HEK-293T and A431 whole cell lysates. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 65 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay play a role in organizing the actin cytoskeleton of the brain cells.
Tissue specificityPredominantly expressed in brain.
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ABP KELCH antibody
- Actin binding protein Mayven antibody
- Actin-binding protein Mayven antibody
All lanes : Anti-KLHL2 antibody (ab227539) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human colon carcinoma tissue stained for KLHL2 with ab227539 at 1/500 dilution in immunohistochemical analysis.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KLHL2 (green) using ab227539 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-alpha-Tubulin antibody at 1/2000 dilution (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227539 has not yet been referenced specifically in any publications.