Key features and details
- Mouse polyclonal to KLHL20
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLHL20 antibody
DescriptionMouse polyclonal to KLHL20
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length KLHL20 protein (Human)
- KLHL20 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).|
FunctionSubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
Cellular localizationCytoplasm > perinuclear region. Nucleus. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination.
- Information by UniProt
- Kelch like ECT2 interacting protein antibody
- Kelch like protein 20 antibody
- Kelch like protein X antibody
All lanes : Anti-KLHL20 antibody (ab67682) at 1/500 dilution
Lane 1 : KLHL20 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
ab67682 has not yet been referenced specifically in any publications.