Key features and details
- Rabbit polyclonal to KLHL25 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KLHL25 antibody - C-terminal
DescriptionRabbit polyclonal to KLHL25 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Rhesus monkey
Recombinant fragment within Human KLHL25 (C terminal). The exact sequence is proprietary.
Database link: Q9H0H3
- WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate. IHC-P: Mouse testis and muscle tissues; Rat fore brain tissue; Human colon carcinoma tissue. ICC/IF: MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
|ICC/IF||1/100 - 1/1000.|
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
- Information by UniProt
- 2810402K13Rik antibody
- BC027373 antibody
- Ectoderm neural cortex protein 2 antibody
Paraffin-embedded mouse testis tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat fore brain tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse muscle tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
Anti-KLHL25 antibody - C-terminal (ab229246) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS-PAGE gel.
Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for KLHL25 (green) using ab229246 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Paraffin-embedded human colon carcinoma tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229246 has not yet been referenced specifically in any publications.