Overview

  • Product name

    Anti-KLHL25 antibody - C-terminal
  • Description

    Rabbit polyclonal to KLHL25 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human KLHL25 (C terminal). The exact sequence is proprietary.
    Database link: Q9H0H3

  • Positive control

    • WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate. IHC-P: Mouse testis and muscle tissues; Rat fore brain tissue; Human colon carcinoma tissue. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Paraffin-embedded mouse testis tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat fore brain tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse muscle tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

  • Anti-KLHL25 antibody - C-terminal (ab229246) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 65 kDa



    7.5% SDS-PAGE gel.

  • Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for KLHL25 (green) using ab229246 at 1/200 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded human colon carcinoma tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

References

ab229246 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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