Key features and details
- Rabbit polyclonal to KLHL3 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KLHL3 antibody - N-terminal
DescriptionRabbit polyclonal to KLHL3 - N-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human KLHL3 (N terminal). The exact sequence is proprietary.
Database link: Q9UH77
- RAW264.7 and 3T3 cell extracts; Human brain tissue; A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
|ICC/IF||1/100 - 1/500.|
RelevanceKLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
Cellular localizationCytoplasmic and cytoskeleton.
- FLJ40871 antibody
- kelch (Drosophila) like 3 antibody
- kelch like 3 (Drosophila) antibody
All lanes : Anti-KLHL3 antibody - N-terminal (ab196776) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : 3T3 cell extract
Lane 3 : 3T3 cell extract with synthesized peptide
Predicted band size: 65 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLHL3 with ab196776 at 1/50 dilution. The image on the right is treated with synthesized peptide.
Immunofluorescent analysis of A549 cells labeling KLHL3 with ab196776 at 1/100 dilution. The image on the right is treated with synthesized peptide.
ab196776 has been referenced in 1 publication.
- Schumacher FR et al. Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia. EMBO Mol Med 7:1285-306 (2015). PubMed: 26286618