Overview

  • Product name

    Anti-KLHL3 antibody - N-terminal
    See all KLHL3 primary antibodies
  • Description

    Rabbit polyclonal to KLHL3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human KLHL3 (N terminal). The exact sequence is proprietary.
    Database link: Q9UH77

  • Positive control

    • RAW264.7 and 3T3 cell extracts; Human brain tissue; A549 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Relevance

    KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
  • Cellular localization

    Cytoplasmic and cytoskeleton.
  • Database links

  • Alternative names

    • FLJ40871 antibody
    • kelch (Drosophila) like 3 antibody
    • kelch like 3 (Drosophila) antibody
    • kelch like 3 antibody
    • Kelch like protein 3 antibody
    • KIAA1129 antibody
    • KLHL 3 antibody
    • KLHL3 antibody
    • MGC44594 antibody
    see all

Images

  • All lanes : Anti-KLHL3 antibody - N-terminal (ab196776) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : 3T3 cell extract
    Lane 3 : 3T3 cell extract with synthesized peptide

    Predicted band size: 65 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLHL3 with ab196776 at 1/50 dilution. The image on the right is treated with synthesized peptide.

  • Immunofluorescent analysis of A549 cells labeling KLHL3 with ab196776 at 1/100 dilution. The image on the right is treated with synthesized peptide.

References

This product has been referenced in:

  • Schumacher FR  et al. Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia. EMBO Mol Med 7:1285-306 (2015). Read more (PubMed: 26286618) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196776.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up