Overview

  • Product name

    Anti-KLHL6 antibody [EPR13484]
    See all KLHL6 primary antibodies
  • Description

    Rabbit monoclonal [EPR13484] to KLHL6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human KLHL6 aa 450-600. The exact sequence is proprietary.
    Database link: Q8WZ60

  • Positive control

    • Human fetal kidney and A549 lysates; A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13484
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/250.
IP 1/50.

Target

  • Function

    Involved in B-lymphocyte antigen receptor signaling and germinal center formation.
  • Tissue specificity

    Found in germinal center B-cells.
  • Sequence similarities

    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • Kelch like protein 6 antibody
    • kelch-like 6 antibody
    • Kelch-like family member 6 antibody
    • Kelch-like protein 6 antibody
    • kelch-like protein KLHL6 antibody
    • KLHL6 antibody
    • KLHL6_HUMAN antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.

  • Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + A549 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

References

This product has been referenced in:

  • Choi J  et al. Loss of KLHL6 promotes diffuse large B-cell lymphoma growth and survival by stabilizing the mRNA decay factor roquin2. Nat Cell Biol 20:586-596 (2018). Read more (PubMed: 29695787) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 22 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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