Overview

  • Product name

    Anti-KLLN antibody [EPR16934]
    See all KLLN primary antibodies
  • Description

    Rabbit monoclonal [EPR16934] to KLLN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLLN aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: B2CW77

  • Positive control

    • HeLa, MCF7, HCT116, Raw264.7, NIH3T3, PC12, C6 whole cell lysates. Human fetal brain and kidney tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function

    DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CWS4 antibody
    • KILIN_HUMAN antibody
    • Killin antibody
    • Killin, p53-regulated DNA replication inhibitor antibody
    • KLLN antibody
    see all

Images

  • All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 20 kDa


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST 

References

This product has been referenced in:

  • Zou A  et al. LINC00472 Acts as a Tumor Suppressor in NSCLC through KLLN-Mediated p53-Signaling Pathway via MicroRNA-149-3p and MicroRNA-4270. Mol Ther Nucleic Acids 17:563-577 (2019). Read more (PubMed: 31382188) »
See 1 Publication for this product

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