Key features and details
- Rabbit polyclonal to Klotho
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Klotho antibody
See all Klotho primary antibodies
DescriptionRabbit polyclonal to Klotho
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- Mouse kidney tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
(or 1/50 - 1/200 using a fluorescent secondary antibody).
|WB||1/100 - 1/1000. Predicted molecular weight: 116 kDa.|
FunctionMay have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Tissue specificityPresent in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
Involvement in diseaseDefects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
Sequence similaritiesBelongs to the glycosyl hydrolase 1 family. Klotho subfamily.
DomainContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
Cellular localizationSecreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
- Information by UniProt
- Kl antibody
- KLOT_HUMAN antibody
- Klotho peptide antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Klotho with ab203576 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Anti-Klotho antibody (ab203576) at 1/100 dilution + 293T cell lysate at 30 µg
IRDye800CW Goat Anti-Rabbit IgG at 1/200000 dilution
Predicted band size: 116 kDa
Additional bands at: 148 kDa (possible glycosylated form)
Klotho has several glycosylation sites, it looks like putative 116 kDa and the glycosylated form around 135 kDa are both correct.
Anti-Klotho antibody (ab203576) at 1/10000 dilution + Mouse kidney lysates at 1/300 dilution
conjugated secondary antibody at 1/10000 dilution
Predicted band size: 116 kDa
ab203576 has been referenced in 12 publications.
- Chen Y et al. Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1. Aging (Albany NY) 12:5651-5674 (2020). PubMed: 32229709
- Kan WC et al. Effect of osthole on advanced glycation end products-induced renal tubular hypertrophy and role of klotho in its mechanism of action. Phytomedicine 53:205-212 (2019). PubMed: 30668400
- Romero A et al. The angiotensin-(1-7)/Mas receptor axis protects from endothelial cell senescence via klotho and Nrf2 activation. Aging Cell 18:e12913 (2019). PubMed: 30773786
- Zhang J et al. Alpha-Klotho, a critical protein for lung health, is not expressed in normal lung. FASEB Bioadv 1:675-687 (2019). PubMed: 32123814
- Liu Y et al. MicroRNA-34a Promotes Renal Fibrosis by Downregulation of Klotho in Tubular Epithelial Cells. Mol Ther 27:1051-1065 (2019). PubMed: 30853453