Overview

  • Product name
    Anti-KMT1A / SUV39H1 antibody
    See all KMT1A / SUV39H1 primary antibodies
  • Description
    Rabbit polyclonal to KMT1A / SUV39H1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 179-412 of Human KMT1A/ SUV39H1 (UniProt O43463).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Raji and mouse brain lysates; HeLa cells; Human Cal27 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
  • Sequence similarities
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Developmental stage
    Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
  • Domain
    Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
    In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
  • Post-translational
    modifications
    Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
    Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
  • Cellular localization
    Nucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • H3 K9 HMTase1 antibody
    • H3-K9-HMTase 1 antibody
    • Histone H3-K9 methyltransferase 1 antibody
    • Histone H3-K9 methyltransferase1 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
    • Histone-lysine N-methyltransferase SUV39H1 antibody
    • KMT1 A antibody
    • KMT1A antibody
    • Lysine N methyltransferase 1A antibody
    • Lysine N-methyltransferase 1A antibody
    • MG44 antibody
    • mIS6 antibody
    • Position-effect variegation 3-9 homolog antibody
    • Su(var)3 9 homolog 1 antibody
    • Su(var)3-9 homolog 1 antibody
    • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
    • Suppressor of variegation 3-9 homolog 1 antibody
    • SUV39 H1 antibody
    • SUV39H antibody
    • SUV39H1 antibody
    • SUV91_HUMAN antibody
    see all

Images

  • All lanes : Anti-KMT1A / SUV39H1 antibody (ab155164) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Anti-KMT1A / SUV39H1 antibody (ab155164) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft tissue labeling KMT1A / SUV39H1 with ab155164 at 1/100 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling KMT1A / SUV39H1 with ab155164 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab155164 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - nuclear (B Cells)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Specification
B Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 27 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 04 2017

Application
Western blot
Sample
Rat Cell lysate - whole cell (Epithelial cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
75 µg
Specification
Epithelial cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 11 2015

Application
ChIP
Sample
Human Cell lysate - other (Epithelial cells)
Negative control
IgG
Specification
Epithelial cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Positive control
Input DNA

Abcam user community

Verified customer

Submitted Sep 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up