Anti-KMT1B / SUV39H2 antibody - C-terminal (ab189842)
Key features and details
- Rabbit polyclonal to KMT1B / SUV39H2 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KMT1B / SUV39H2 antibody - C-terminal
See all KMT1B / SUV39H2 primary antibodies -
Description
Rabbit polyclonal to KMT1B / SUV39H2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Cynomolgus monkey, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human KMT1B/ SUV39H2 aa 100-350.
Database link: Q9H5I1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 47 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa. |
Target
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Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Domain
Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. -
Cellular localization
Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 426314 Chicken
- Entrez Gene: 536936 Cow
- Entrez Gene: 102136014 Cynomolgus monkey
- Entrez Gene: 79723 Human
- Entrez Gene: 64707 Mouse
- Entrez Gene: 549262 Xenopus tropicalis
- Omim: 606503 Human
- SwissProt: Q5F3W5 Chicken
see all -
Alternative names
- FLJ23414 antibody
- H3 K9 HMTase 2 antibody
- H3-K9-HMTase 2 antibody
see all
Images
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All lanes : Anti-KMT1B / SUV39H2 antibody - C-terminal (ab189842) at 1/1000 dilution
Lane 1 : SW620 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Mouse heart tissue lysate
Lane 7 : Mouse liver tissue lysate
Lane 8 : Mouse eye tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 47 kDaBlocking buffer: 3% nonfat dry milk in TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab189842 has been referenced in 1 publication.
- Guerra MV et al. H3K9 Methyltransferases Suv39h1 and Suv39h2 Control the Differentiation of Neural Progenitor Cells in the Adult Hippocampus. Front Cell Dev Biol 9:778345 (2021). PubMed: 35096813