Key features and details
- Rabbit polyclonal to KMT1B / SUV39H2 - C-terminal
- Suitable for: WB, ChIP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KMT1B / SUV39H2 antibody - C-terminal
See all KMT1B / SUV39H2 primary antibodies
DescriptionRabbit polyclonal to KMT1B / SUV39H2 - C-terminal
Tested applicationsSuitable for: WB, ChIPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Cynomolgus monkey, Xenopus tropicalis
Recombinant fragment corresponding to Human KMT1B/ SUV39H2 aa 141-350.
CCPAEAGVLLAYNKNQQIKIPPGTPIYECNSRCQCGPDCPNRIVQKGTQY SLCIFRTSNGRGWGVKTLVKIKRMSFVMEYVGEVITSEEAERRGQFYDNK GITYLFDLDYESDEFTVDAARYGNVSHFVNHSCDPNLQVFNVFIDNLDTR LPRIALFSTRTINAGEELTFDYQMKGSGDISSDSIDHSPAKKRVRTVCKC GAVTCRGYLN
Database link: Q9H5I1
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 47 kDa.|
|ChIP||1/20 - 1/100.|
FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
DomainAlthough the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
Cellular localizationNucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
- Information by UniProt
- FLJ23414 antibody
- H3 K9 HMTase 2 antibody
- H3-K9-HMTase 2 antibody
All lanes : Anti-KMT1B / SUV39H2 antibody - C-terminal (ab189842) at 1/1000 dilution
Lane 1 : SW620 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Mouse heart tissue lysate
Lane 7 : Mouse liver tissue lysate
Lane 8 : Mouse eye tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 47 kDa
Blocking buffer: 3% nonfat dry milk in TBST.
Chromatin immunoprecipitation analysis of extracts of 293T cell line, using ab189842 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ab189842 has not yet been referenced specifically in any publications.