Key features and details
- Mouse monoclonal [5H6A12] to KMT1E / SETDB1
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG1
Product nameAnti-KMT1E / SETDB1 antibody [5H6A12]
See all KMT1E / SETDB1 primary antibodies
DescriptionMouse monoclonal [5H6A12] to KMT1E / SETDB1
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Monkey
Purified recombinant fragment of human SETDB1 expressed in E. Coli.
- MCF-7,T47D, HEK293, JURKAT, NIH/3T3, F9, RAW246.7 and COS-7 cell lysates.
This product was changed from ascites to supernatant. Lot no’s high than GR223392-13 are from Tissue Culture Supernatant
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab107225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 180 kDa.|
|ICC/IF||1/200 - 1/1000.|
FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificityWidely expressed. High expression in testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
DomainThe pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localizationNucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
- Information by UniProt
- AU022152 antibody
- EC 184.108.40.206 antibody
- ERG-associated protein with SET domain antibody
All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution
Lane 1 : MCF-7
Lane 2 : T47D
Lane 3 : HEK293
Lane 4 : JURKAT
Lane 5 : NIH/3T3
Lane 6 : F9
Lane 7 : RAW264.7
Lane 8 : COS-7
Predicted band size: 180 kDa
Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).
Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab107225 has been referenced in 1 publication.
- Ropa J et al. PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of Hoxa9 and Meis1 in acute myeloid leukemia. Oncotarget 9:22123-22136 (2018). PubMed: 29774127