Overview

  • Product name

    Anti-KMT1E / SETDB1 antibody
    See all KMT1E / SETDB1 primary antibodies
  • Description

    Rabbit polyclonal to KMT1E / SETDB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ChIPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human KMT1E/ SETDB1 (intracellular). The exact sequence is proprietary.
    Database link: Q15047

  • Positive control

    • ChIP: HeLa chromatin extract. IP: HeLa nuclear extract. WB: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 143 kDa.
IP 1/100 - 1/500.
ChIP Use at an assay dependent concentration.

Target

  • Function

    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • Tissue specificity

    Widely expressed. High expression in testis.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • Domain

    The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • Cellular localization

    Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • AU022152 antibody
    • EC 2.1.1.43 antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Images

  • Anti-KMT1E / SETDB1 antibody (ab228575) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 143 kDa



    5% SDS-PAGE

  • KMT1E / SETDB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract using 4 µg of ab228575. Western blot was performed from the immunoprecipitate using ab228575 at 1/1000 dilution.

    Lane 1: Control IgG IP in HeLa nuclear extract.

    Lane 2: ab228575 IP in HeLa nuclear extract.

    5% SDS-PAGE

  • Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228575. The precipitated DNA was detected by PCR with primer set targeting to p53P2.

References

ab228575 has not yet been referenced specifically in any publications.

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