Product nameAnti-KMT1E / SETDB1 antibody
See all KMT1E / SETDB1 primary antibodies
DescriptionRabbit polyclonal to KMT1E / SETDB1
Tested applicationsSuitable for: WB, IP, ChIPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment within Human KMT1E/ SETDB1 (internal sequence). The exact sequence is proprietary.
Database link: Q15047
- ChIP: HeLa chromatin extract. WB: U-87 MG whole cell lysate. IP: HeLa nuclear extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 143 kDa.|
|IP||1/100 - 1/500.|
|ChIP||Use at an assay dependent concentration.|
FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificityWidely expressed. High expression in testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
DomainThe pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localizationNucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
- Information by UniProt
- AU022152 antibody
- EC 184.108.40.206 antibody
- ERG-associated protein with SET domain antibody
Anti-KMT1E / SETDB1 antibody (ab228984) at 1/2000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 143 kDa
Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228984. The precipitated DNA was detected by PCR with a primer set targeting to P53P2.
ab228984 immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract.
Lane 1: Control IP with 4 μg of preimmune Rabbit IgG.
Lane 2: Immunoprecipitation of SETDB1 protein by 4 μg ab228984.
The immunoprecipitated SETDB1 protein was detected by ab228984 at 1/1000 dilution.
5 % SDS-PAGE
ab228984 has not yet been referenced specifically in any publications.