Key features and details
- Rabbit polyclonal to KMT1E / SETDB1
- Suitable for: WB, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KMT1E / SETDB1 antibody
See all KMT1E / SETDB1 primary antibodies
DescriptionRabbit polyclonal to KMT1E / SETDB1
Tested applicationsSuitable for: WB, IP, ChIPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment within Human KMT1E/ SETDB1 (internal sequence). The exact sequence is proprietary.
Database link: Q15047
- ChIP: HeLa chromatin extract. WB: U-87 MG whole cell lysate. IP: HeLa nuclear extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 143 kDa.|
|IP||1/100 - 1/500.|
|ChIP||Use at an assay dependent concentration.|
FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificityWidely expressed. High expression in testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
DomainThe pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localizationNucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
- Information by UniProt
- AU022152 antibody
- EC 184.108.40.206 antibody
- ERG-associated protein with SET domain antibody
Anti-KMT1E / SETDB1 antibody (ab228984) at 1/2000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 143 kDa
Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228984. The precipitated DNA was detected by PCR with a primer set targeting to P53P2.
ab228984 immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract.
Lane 1: Control IP with 4 μg of preimmune Rabbit IgG.
Lane 2: Immunoprecipitation of SETDB1 protein by 4 μg ab228984.
The immunoprecipitated SETDB1 protein was detected by ab228984 at 1/1000 dilution.
5 % SDS-PAGE
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228984 has not yet been referenced specifically in any publications.