Product nameAnti-KMT2B / MLL4 antibody
See all KMT2B / MLL4 primary antibodies
DescriptionGoat polyclonal to KMT2B / MLL4
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset
- IHC-P: Human brain cortex and testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityProtein G purified
Purification notesImmunoaffinity purified
Our Abpromise guarantee covers the use of ab104444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration.|
FunctionHistone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
Tissue specificityWidely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
Contains 3 A.T hook DNA-binding domains.
Contains 1 CXXC-type zinc finger.
Contains 1 FYR C-terminal domain.
Contains 1 FYR N-terminal domain.
Contains 3 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 1 SET domain.
- Information by UniProt
- Histone-lysine N-methyltransferase 2B antibody
- HRX2 antibody
- KIAA0304 gene product antibody
ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
This product has been referenced in:
- Su CH et al. Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification. PLoS One 11:e0166090 (2016). ChIP, IHC-P ; Human . Read more (PubMed: 27806114) »
- Fulcoli FG et al. Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Nat Commun 7:11688 (2016). Read more (PubMed: 27256596) »