Western blot abreview for Anti-KMT2B / MLL4 antibody

Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (5%)
Sample
Mouse Cell lysate - whole cell (primary bone marrow derived macrophages)
Specification
primary bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Polyclonal Rabbit Anti-Goat dakocytomation
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Clarity ECL biorad
Bands
Specific: >250 kDa
Exposure
20 minute(s) and 0 second(s)

Additional data

Abcam response
We are happy that this antibody worked well for this customer in the new species and application. The customer participate in our AbTrial program and will use the discount code for the purchase of any product on the next Abcam order. However, since mouse and WB were not previously tested, we would like to receive further feedback from other customers that used the antibody in this species and/or application. Please contact our Scientific Support for further information.

Abcam user community

Verified customer

Submitted Sep 10 2014

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