Western blot abreview for Anti-KMT2B / MLL4 antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
100000 cells
Specification
mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: can get signal
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti goat IgG
Host species: Guinea pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
Whole cell extract of KMt2b knockout eHAP1 cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 80 kDa Non-specific: 120, 55, 40, 35 kDa
Positive control
Whole cell extract of eHAP1 cells

Additional data

Additional Notes
It works for WB. We detected a specific band at 80 kDa, which is supposed to be a cleaved form of Ktm2b, in wild type but not in Kmt2b knockout cells.

Abcam user community

Verified customer

Submitted Aug 17 2018

Sign up