Immunocytochemistry/ Immunofluorescence abreview for Anti-KMT2B / MLL4 antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cells)
Permeabilization
Yes - 0.1% TritonX-100
Specification
mouse ES cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Concentration
5 µg/ml

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: anti-Goat IgG Alexa555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
We found that it did not work for immunostaining on mouse ES cells. We detected weak signals not only in nucleus but also in cytoplasm, which is presumably background signals. Furthermore, we got the same results with a negative control, Kmt2b knock-out ES cells.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

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Verified customer

Submitted Jan 04 2019

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