Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab15962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionHistone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
Tissue specificityExpressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.
Involvement in diseaseDefects in MLL2 are the cause of Kabuki syndrome (KABS) [MIM:147920]. It is a congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. TRX/MLL subfamily.
Contains 1 FY-rich C-terminal domain.
Contains 1 FY-rich N-terminal domain.
Contains 5 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 4 RING-type zinc fingers.
Contains 1 SET domain.
DomainLXXLL motifs 5 and 6 are essential for the association with ESR1 nuclear receptor.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- AAD10 antibody
- ALL1 related gene antibody
- ALL1-related protein antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining KMT2D / MLL2 with ab15962 at 4 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining KMT2D / MLL2 with ab15962 at 3.8 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).
ab15962 has been referenced in 3 publications.
- Abudureheman A et al. High MLL2 expression predicts poor prognosis and promotes tumor progression by inducing EMT in esophageal squamous cell carcinoma. J Cancer Res Clin Oncol 144:1025-1035 (2018). PubMed: 29532228
- Pradeepa MM et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res 42:9021-32 (2014). ChIP . PubMed: 25056311
- Patel SR et al. The BRCT-domain containing protein PTIP links PAX2 to a histone H3, lysine 4 methyltransferase complex. Dev Cell 13:580-92 (2007). PubMed: 17925232