Anti-KMT2D / MLL2 antibody (ab231239)
Key features and details
- Rabbit polyclonal to KMT2D / MLL2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-KMT2D / MLL2 antibody
See all KMT2D / MLL2 primary antibodies -
Description
Rabbit polyclonal to KMT2D / MLL2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse KMT2D/ MLL2 (internal sequence) conjugated to keyhole limpet haemocyanin. Three peptides.
Database link: Q6PDK2 -
Positive control
- WB: Mouse embryonic stem cell (E14Tg2a) whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500.
|
Notes |
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WB
1/500. |
Target
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Function
Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation. -
Tissue specificity
Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver. -
Involvement in disease
Defects in MLL2 are the cause of Kabuki syndrome (KABS) [MIM:147920]. It is a congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily.
Contains 1 FY-rich C-terminal domain.
Contains 1 FY-rich N-terminal domain.
Contains 5 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 4 RING-type zinc fingers.
Contains 1 SET domain. -
Domain
LXXLL motifs 5 and 6 are essential for the association with ESR1 nuclear receptor. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 381022 Mouse
- SwissProt: Q6PDK2 Mouse
- Unigene: 264889 Mouse
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Alternative names
- AAD10 antibody
- ALL1 related gene antibody
- ALL1-related protein antibody
see all
Images
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All lanes : Anti-KMT2D / MLL2 antibody (ab231239) at 1/500 dilution
Lane 1 : Mouse embryonic stem cells (E14Tg2a) whole cell lysate (Heterozygous knockout)
Lanes 2-3 : Mouse embryonic stem cells (E14Tg2a) whole cell lysate (Homozygous knockout)
Lane 4 : Mouse embryonic stem cells (E14Tg2a) whole cell lysate (flp recombined)Cells homozygous for the targeted KMT2D / MLL2 allele (-/-) show a complete loss of KMT2D / MLL2 protein, whereas the KMT2D / MLL2 protein was detected in fp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).
Dilution buffer: PBS-Tween containing 5% skimmed milk.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab231239 has been referenced in 1 publication.
- Li M et al. Histone Methyltransferase KMT2D Regulates H3K4 Methylation and is Involved in the Pathogenesis of Ovarian Cancer. Cell Transplant 30:9636897211027521 (2021). PubMed: 34705580