Our Abpromise guarantee covers the use of ab15962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.


  • Function

    Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
  • Tissue specificity

    Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.
  • Involvement in disease

    Defects in MLL2 are the cause of Kabuki syndrome (KABS) [MIM:147920]. It is a congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily.
    Contains 1 FY-rich C-terminal domain.
    Contains 1 FY-rich N-terminal domain.
    Contains 5 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 4 RING-type zinc fingers.
    Contains 1 SET domain.
  • Domain

    LXXLL motifs 5 and 6 are essential for the association with ESR1 nuclear receptor.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • AAD10 antibody
    • ALL1 related gene antibody
    • ALL1-related protein antibody
    • ALR antibody
    • CAGL114 antibody
    • Histone-lysine N-methyltransferase MLL2 antibody
    • KABUK1 antibody
    • Kabuki make up syndrome antibody
    • Kabuki mental retardation syndrome antibody
    • KMS antibody
    • KMT2B antibody
    • KMT2D antibody
    • Lysine N methyltransferase 2D antibody
    • Lysine N-methyltransferase 2B antibody
    • MLL2 antibody
    • MLL2_HUMAN antibody
    • MLL4 antibody
    • Myeloid/lymphoid or mixed lineage leukemia 2 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia protein 2 antibody
    • TNRC21 antibody
    • Trinucleotide repeat containing 21 antibody
    see all


  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining KMT2D / MLL2 with ab15962 at 4 &microg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining KMT2D / MLL2 with ab15962 at 3.8 &microg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).


This product has been referenced in:

  • Abudureheman A  et al. High MLL2 expression predicts poor prognosis and promotes tumor progression by inducing EMT in esophageal squamous cell carcinoma. J Cancer Res Clin Oncol 144:1025-1035 (2018). Read more (PubMed: 29532228) »
  • Pradeepa MM  et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res 42:9021-32 (2014). ChIP . Read more (PubMed: 25056311) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Human Cell lysate - nuclear (K562 (human erythroid cell line))
Negative control
Normal IgG
K562 (human erythroid cell line)
Detection step
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
GATA-1 antibody

Abcam user community

Verified customer

Submitted Oct 20 2015


The antibody incubation time and temperature was 1h at ambient temp for IHC-P - for both images.

Read More


Thank you for your email. As far as I know, there are currently no plans for production of a MLL4 antibody. I suggest checking back with us in the future, and if you have any additional questions, please do let us know.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up