Key features and details
- Rabbit polyclonal to KMT3A / HYPB / HIF-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KMT3A / HYPB / HIF-1 antibody
See all KMT3A / HYPB / HIF-1 primary antibodies
DescriptionRabbit polyclonal to KMT3A / HYPB / HIF-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human KMT3A/ HYPB/ HIF-1 aa 453-679. The exact sequence is proprietary.
Database link: Q9BYW2
- Raji and K562 whole cell lysates; HBL435 xenograft tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab184190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 288 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionHistone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 1 post-SET domain.
Contains 1 SET domain.
Contains 1 WW domain.
DomainThe low charge region mediates the transcriptional activation activity.
modificationsMay be automethylated.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- EC 220.127.116.11 antibody
- FLJ16420 antibody
- FLJ22472 antibody
All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab184190) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 288 kDa
5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft tissue, labeling KMT3A / HYPB / HIF-1 with ab184190 at 1/500 dilution.
ab184190 has been referenced in 1 publication.
- Liu L et al. Loss of SETD2, but not H3K36me3, correlates with aggressive clinicopathological features of clear cell renal cell carcinoma patients. Biosci Trends 11:214-220 (2017). PubMed: 28260718