Product nameAnti-KMT3A / HYPB / HIF-1 antibody
See all KMT3A / HYPB / HIF-1 primary antibodies
DescriptionRabbit polyclonal to KMT3A / HYPB / HIF-1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in the following whole cell lysates: HeLa; MDA-MB-231.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 288 kDa (predicted molecular weight: 288 kDa).|
FunctionHistone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 1 post-SET domain.
Contains 1 SET domain.
Contains 1 WW domain.
DomainThe low charge region mediates the transcriptional activation activity.
modificationsMay be automethylated.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- EC 188.8.131.52 antibody
- FLJ16420 antibody
- FLJ22472 antibody
All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab31358) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 288 kDa
Observed band size: 288 kDa
Additional bands at: 110 kDa, 130 kDa, 30 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31358 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
This product has been referenced in:
- Ji H et al. The Effect of Dry Eye Disease on Scar Formation in Rabbit Glaucoma Filtration Surgery. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28555041) »
- Papillon-Cavanagh S et al. Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas. Nat Genet 49:180-185 (2017). Read more (PubMed: 28067913) »