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I have ordered antibodies ab31358, ab69836,ab89432
and wondered if you recommended blocking and probing in 5% BSA or 5% milk?
Asked on May 09 2012
Thank you for your inquiry.
Most antibodies will work fine with either BSA or milk.
When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend this.
Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands.
An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : https://www.abcam.com/ab9385 .
Unfortunately, this has to be tested experimentally according to your experimental set-up. But I suggest top start with BSA and only change the blocking agent when there is need.
I hope this information is helpful and wish you good luck with your experiments.
Answered on May 09 2012