Anti-KMT3C / SMYD2 antibody (ab234862)
Key features and details
- Rabbit polyclonal to KMT3C / SMYD2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT3C / SMYD2 antibody
See all KMT3C / SMYD2 primary antibodies -
Description
Rabbit polyclonal to KMT3C / SMYD2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human KMT3C/ SMYD2 aa 100-300.
Database link: Q9NRG4 -
Positive control
- IHC-P: Human testis and small intestine tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. -
Sequence similarities
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
Cellular localization
Cytoplasm > cytosol. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 421361 Chicken
- Entrez Gene: 615229 Cow
- Entrez Gene: 56950 Human
- Entrez Gene: 226830 Mouse
- Entrez Gene: 100294706 Pig
- Entrez Gene: 289372 Rat
- Omim: 610663 Human
- SwissProt: E1C5V0 Chicken
see all -
Alternative names
- Histone methyltransferase SMYD2 antibody
- HSKM B antibody
- HSKM-B antibody
see all
Images
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KMT3C / SMYD2 (green) using ab234862 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human testis tissue stained for KMT3C / SMYD2 using ab234862 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human small intestine tissue stained for KMT3C / SMYD2 using ab234862 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab234862 has been referenced in 1 publication.
- Sun JJ et al. SMYD2 promotes cervical cancer growth by stimulating cell proliferation. Cell Biosci 9:75 (2019). PubMed: 31548876