Anti-KMT3C / SMYD2 antibody (ab108217)
Key features and details
- Rabbit polyclonal to KMT3C / SMYD2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KMT3C / SMYD2 antibody
See all KMT3C / SMYD2 primary antibodies -
Description
Rabbit polyclonal to KMT3C / SMYD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T and NIH 3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab108217 was affinity purified using an epitope specific to KMT3C / SMYD2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. -
Sequence similarities
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
Cellular localization
Cytoplasm > cytosol. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 421361 Chicken
- Entrez Gene: 615229 Cow
- Entrez Gene: 56950 Human
- Entrez Gene: 226830 Mouse
- Entrez Gene: 100294706 Pig
- Omim: 610663 Human
- SwissProt: E1C5V0 Chicken
- SwissProt: Q0P585 Cow
see all -
Alternative names
- Histone methyltransferase SMYD2 antibody
- HSKM B antibody
- HSKM-B antibody
see all
Images
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All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 0.1 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes -
All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 1/1000 dilution
Lane 1 : U2OS whole cell lysate - siRNA targeting luciferase
Lanes 2-3 : U2OS whole cell lysate - siRNA targeting SMYD2
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 20 secondsBlocked with 5% BSA for 1 hour at room temperature.
Primary antibody incubation time: 1 hour.
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Detection of KMT3C / SMYD2 by Western blot of Immunprecipitate.
ab108217, at 1 µg/ml, staining KMT3C / SMYD2 in HeLa whole cell lysate immunoprecipitated using ab108217 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab108217 has been referenced in 4 publications.
- Sun JJ et al. SMYD2 promotes cervical cancer growth by stimulating cell proliferation. Cell Biosci 9:75 (2019). PubMed: 31548876
- Eggert E et al. Discovery and Characterization of a Highly Potent and Selective Aminopyrazoline-Based in Vivo Probe (BAY-598) for the Protein Lysine Methyltransferase SMYD2. J Med Chem 59:4578-600 (2016). PubMed: 27075367
- Xu G et al. The histone methyltransferase Smyd2 is a negative regulator of macrophage activation by suppressing interleukin 6 (IL-6) and tumor necrosis factor a (TNF-a) production. J Biol Chem 290:5414-23 (2015). ChIP ; Mouse . PubMed: 25583990
- Liu T et al. Master redox regulator Trx1 upregulates SMYD1 & modulates lysine methylation. Biochim Biophys Acta 1854:1816-22 (2015). WB ; Human . PubMed: 26410624