Anti-KMT3C / SMYD2 antibody (ab154749)
Key features and details
- Rabbit polyclonal to KMT3C / SMYD2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-KMT3C / SMYD2 antibody
See all KMT3C / SMYD2 primary antibodies -
Description
Rabbit polyclonal to KMT3C / SMYD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 115-310 of Human KMT3C/ SMYD2 (UniProt ID: Q9NRG4).
-
Positive control
- A549, HeLa, HepG2 or HCT116 whole cell lysate; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. -
Sequence similarities
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
Cellular localization
Cytoplasm > cytosol. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 615229 Cow
- Entrez Gene: 56950 Human
- Entrez Gene: 226830 Mouse
- Entrez Gene: 100294706 Pig
- Entrez Gene: 289372 Rat
- Omim: 610663 Human
- SwissProt: Q0P585 Cow
- SwissProt: Q9NRG4 Human
see all -
Alternative names
- Histone methyltransferase SMYD2 antibody
- HSKM B antibody
- HSKM-B antibody
see all
Images
-
All lanes : Anti-KMT3C / SMYD2 antibody (ab154749) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cell, labeling KMT3C / SMYD2 using ab154749 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154749 has not yet been referenced specifically in any publications.