Product nameAnti-KMT4 / Dot1L antibody
See all KMT4 / Dot1L primary antibodies
DescriptionRabbit polyclonal to KMT4 / Dot1L
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Orangutan
Synthetic peptide (Human) corresponding to a region between residues 1000 and 1050 of KMT4/ Dot1L. (NP_115871.1).
- Whole cell HeLa lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionHistone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Sequence similaritiesBelongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
Contains 1 DOT1 domain.
- Information by UniProt
- Disrupter of telomere silencing protein 1 antibody
- DOT 1 antibody
- DOT1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KMT4 / Dot1L with ab72454 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-KMT4 / Dot1L antibody (ab72454) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 185 kDa
Observed band size: 185 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
HeLa whole cell lysate ( 1 mg for IP, 20% of IP loaded) with ab72454 at 3 µg/mg lysate for IP, followed by ab72454 at 1 µg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Nassa G et al. Inhibition of histone methyltransferase DOT1L silences ERa gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv 5:eaav5590 (2019). Read more (PubMed: 30775443) »
- Liu Y et al. Nuclear lactate dehydrogenase A senses ROS to produce a-hydroxybutyrate for HPV-induced cervical tumor growth. Nat Commun 9:4429 (2018). Read more (PubMed: 30356100) »