Key features and details
- Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KMT5A / SETD8 / Pr-SET7 antibody
See all KMT5A / SETD8 / Pr-SET7 primary antibodies
DescriptionRabbit polyclonal to KMT5A / SETD8 / Pr-SET7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 81-290 of Human KMT5A/ SETD8/ Pr-SET7, isoform 1(42kda) and 2(39KDa)
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; HeLa xenograft tissue.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab111691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProtein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. PR/SET subfamily.
Contains 1 SET domain.
Developmental stageNot detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
DomainAlthough the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Cellular localizationNucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
- Information by UniProt
- H4 K20 HMTase antibody
- H4 K20 specific histone methyltransferase antibody
- H4-K20-HMTase SETD8 antibody
ab111691 has not yet been referenced specifically in any publications.