Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (fibroblast)
Specification
fibroblast
Treatment
siRNA for 48hrs
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 40 kDa Non-specific: 76, 74, 70 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you so much for testing this antibody in mouse.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species. We would welcome additional feedback from other customers as well.
Abcam user community
Verified customer
Submitted Jul 09 2013