Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 52, 60, 61 kDa
Additional data
Abcam response
Thank you for submitting this AbReview and your valuable feedback about the use of ab111691 in mouse, a species we have not tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in mouse which will help us assess how well the antibody works in this species.
Mr. Brian Koss
Verified customer
Submitted Oct 23 2018