Overview

  • Product name

    Anti-KMT5A / SETD8 / Pr-SET7 antibody
    See all KMT5A / SETD8 / Pr-SET7 primary antibodies
  • Description

    Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein (His-DDDDK-tag) corresponding to Human KMT5A/ SETD8/ Pr-SET7. (DDDDK-tagged N-terminus and His-tagged C terminus).
    Database link: Q9NQR1

  • Positive control

    • WB: HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab230683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
    Contains 1 SET domain.
  • Developmental stage

    Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
  • Domain

    Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
  • Cellular localization

    Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • H4 K20 HMTase antibody
    • H4 K20 specific histone methyltransferase antibody
    • H4-K20-HMTase SETD8 antibody
    • Histone H4 K20 methyltransferase antibody
    • Histone lysine N methyltransferase H4 lysine 20 specific antibody
    • Histone-lysine N-methyltransferase SETD8 antibody
    • Lysine N-methyltransferase 5A antibody
    • N-lysine methyltransferase SETD8 antibody
    • Pr SET 7 antibody
    • Pr SET7 antibody
    • PR-Set7 antibody
    • PR/SET domain containing protein 07 antibody
    • PR/SET domain containing protein 8 antibody
    • PR/SET domain-containing protein 07 antibody
    • PR/SET07 antibody
    • PrSET7 antibody
    • SET 07 antibody
    • SET 8 antibody
    • SET domain containing (lysine methyltransferase) 8 antibody
    • SET domain containing 8 antibody
    • SET domain containing lysine methyltransferase 8 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 8 antibody
    • SET07 antibody
    • SET8 antibody
    • SETD 8 antibody
    • setd8 antibody
    • SETD8_HUMAN antibody
    see all

Images

  • Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab230683) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract at 20 µg

    Predicted band size: 42 kDa

References

ab230683 has not yet been referenced specifically in any publications.

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