Key features and details
- Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KMT5A / SETD8 / Pr-SET7 antibody
See all KMT5A / SETD8 / Pr-SET7 primary antibodies
DescriptionRabbit polyclonal to KMT5A / SETD8 / Pr-SET7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant full length protein (His-DDDDK-tag) corresponding to Human KMT5A/ SETD8/ Pr-SET7. (DDDDK-tagged N-terminus and His-tagged C terminus).
Database link: Q9NQR1
- WB: HeLa nuclear extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab230683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 42 kDa.|
FunctionProtein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. PR/SET subfamily.
Contains 1 SET domain.
Developmental stageNot detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
DomainAlthough the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Cellular localizationNucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
- Information by UniProt
- H4 K20 HMTase antibody
- H4 K20 specific histone methyltransferase antibody
- H4-K20-HMTase SETD8 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230683 has not yet been referenced specifically in any publications.