Overview

  • Product name
    Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)]
    See all KMT5A / SETD8 / Pr-SET7 primary antibodies
  • Description
    Rabbit monoclonal [EPR11321(2)] to KMT5A / SETD8 / Pr-SET7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KMT5A/ SETD8/ Pr-SET7 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NQR1

  • Positive control
    • WB: 293T, Saos-2, HCT-116 and U87-MG cell lysates; FC: 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
    • Sequence similarities
      Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
      Contains 1 SET domain.
    • Developmental stage
      Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
    • Domain
      Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
    • Cellular localization
      Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • H4 K20 HMTase antibody
      • H4 K20 specific histone methyltransferase antibody
      • H4-K20-HMTase SETD8 antibody
      • Histone H4 K20 methyltransferase antibody
      • Histone lysine N methyltransferase H4 lysine 20 specific antibody
      • Histone-lysine N-methyltransferase SETD8 antibody
      • Lysine N-methyltransferase 5A antibody
      • N-lysine methyltransferase SETD8 antibody
      • Pr SET 7 antibody
      • Pr SET7 antibody
      • PR-Set7 antibody
      • PR/SET domain containing protein 07 antibody
      • PR/SET domain containing protein 8 antibody
      • PR/SET domain-containing protein 07 antibody
      • PR/SET07 antibody
      • PrSET7 antibody
      • SET 07 antibody
      • SET 8 antibody
      • SET domain containing (lysine methyltransferase) 8 antibody
      • SET domain containing 8 antibody
      • SET domain containing lysine methyltransferase 8 antibody
      • SET domain containing protein 8 antibody
      • SET domain-containing protein 8 antibody
      • SET07 antibody
      • SET8 antibody
      • SETD 8 antibody
      • setd8 antibody
      • SETD8_HUMAN antibody
      see all

    Images

    • All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)] (ab177488) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Rat kidney lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)] (ab177488) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Rat kidney lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    • All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)] (ab177488) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse Kidney lysate
      Lane 4 : Mouse spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    • All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)] (ab177488) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : HCT-116 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Flow Cytometrical analysis of permeabilized 293T cells labeling KMT5A / SETD8 / Pr-SET7 with ab177488 at 1/100 (red) or a rabbit IgG (negative) (green).

    References

    ab177488 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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