Rabbit monoclonal [EPR11321(2)] to KMT5A / SETD8 / Pr-SET7
Flow Cyt, WBmore details Unsuitable for:
ICC/IF,IHC-P or IP
Mouse, Rat, Human
Synthetic peptide within Human KMT5A/ SETD8/ Pr-SET7 aa 50-150 (Cysteine residue). The exact sequence is proprietary. Database link: Q9NQR1
293T, Saos-2, HCT-116 and U87-MG cell lysates; 293T cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
Is unsuitable for ICC/IF,IHC-P or IP.
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Contains 1 SET domain.
Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.