Overview

  • Product name

    Anti-KMT5C/SUV4-20h2 antibody
  • Description

    Rabbit polyclonal to KMT5C/SUV4-20h2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the center region of Human KMT5C/SUV4-20h2 conjugated to KLH

  • Positive control

    • Mouse stomach tissue lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    Following purification, this antibody was dialysed against PBS
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 52 kDa.
ELISA 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 24459145

Target

  • Function

    Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).
  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus. Chromosome. Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ98627 antibody
    • Histone lysine N methyltransferase SUV420H2 antibody
    • Histone-lysine N-methyltransferase SUV420H2 antibody
    • KMT5C antibody
    • Lysine N methyltransferase 5C antibody
    • Lysine N-methyltransferase 5C antibody
    • MGC2705 antibody
    • Su(var)4 20 homolog 2 antibody
    • Su(var)4-20 homolog 2 antibody
    • Suppressor of variegation 4 20 homolog 2 antibody
    • Suppressor of variegation 4-20 homolog 2 antibody
    • Suv4 20h2 antibody
    • Suv4-20h2 antibody
    • SUV420H2 antibody
    • SV422_HUMAN antibody
    see all

Images

  • Anti-KMT5C/SUV4-20h2 antibody (ab91224) at 1/50 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size: 52 kDa

  • Chromatin from cells was immunoprecipitated with IgG (negative control) or SUV420H2 antibodies (ab91224), and DNA was extracted from the immunoprecipitated samples. Immunoprecipitated DNA was subjected to real-time PCR with primer sets that span the TMS1 genomic regions and is expressed as a percentage of input DNA. Data are mean ± S.D. from a representative experiment from triplicate determinants.

References

This product has been referenced in:

  • Lyu G  et al. TGF-ß signaling alters H4K20me3 status via miR-29 and contributes to cellular senescence and cardiac aging. Nat Commun 9:2560 (2018). Read more (PubMed: 29967491) »
  • Khani F  et al. Histone H4 Methyltransferase Suv420h2 Maintains Fidelity of Osteoblast Differentiation. J Cell Biochem 118:1262-1272 (2017). Read more (PubMed: 27862226) »
See all 5 Publications for this product

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