Anti-KMT6 / EZH2 antibody (ab186006)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KMT6 / EZH2 antibody
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit polyclonal to KMT6 / EZH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human KMT6/ EZH2 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. CAA64955.1
Database link: Q15910 -
Positive control
- 293T, HeLa, Jurkat, and Mouse TCMK-1 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/2500. Predicted molecular weight: 85 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2500. Predicted molecular weight: 85 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. -
Tissue specificity
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
Developmental stage
Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. -
Post-translational
modificationsPhosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 509106 Cow
- Entrez Gene: 475511 Dog
- Entrez Gene: 2146 Human
- Entrez Gene: 14056 Mouse
- Entrez Gene: 100625497 Pig
- Entrez Gene: 100345157 Rabbit
- Entrez Gene: 709075 Rhesus monkey
- Entrez Gene: 100381148 Xenopus laevis
see all -
Alternative names
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
see all
Images
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Lane 1 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg
Lanes 2-4 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 cells.lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 3 minutes -
Mouse plasmacytoma tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Human ovarian tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Detection of KMT6 / EZH2 by Western Blot of Immunprecipitate.
Lane 1: ab186006 at 1 µg/ml labeling KMT6 / EZH2 in 293T whole cell lysate immunoprecipitated using ab186006 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab186006 has been referenced in 70 publications.
- Niwa Y et al. Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway. J Clin Med 11:N/A (2022). PubMed: 35160302
- Yoon JH et al. lncRNAs UC.145 and PRKG1-AS1 Determine the Functional Output of DKK1 in Regulating the Wnt Signaling Pathway in Gastric Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35625973
- Xiang F & Xu X CirRNA F-circEA-2a Suppresses the Role of miR-3613-3p in Colorectal Cancer by Direct Sponging and Predicts Poor Survival. Cancer Manag Res 14:1825-1833 (2022). PubMed: 35652063
- Chen T et al. LncRNA Airn maintains LSEC differentiation to alleviate liver fibrosis via the KLF2-eNOS-sGC pathway. BMC Med 20:335 (2022). PubMed: 36171606
- Chen YN et al. EZH2 is a potential prognostic predictor of glioma. J Cell Mol Med 25:925-936 (2021). PubMed: 33277782